The Survey on Antimicrobial Effects of Methanolic Extract of Carum Copticum L. on Staphylococcus Aureus, Bacillus Cereus, Escherichia Coli and Pseudomonas Aeruginosa in Laboratory Conditions

Introduction: Nowadays, the prevalence of drug resistance to synthetic drugs is increasing in many countries in the world; therefore, many efforts have been made to find new compounds as an appropriate alternative to antibiotics. This study was performed to investigate the antibacterial effect of methanolic extract of Carum copticum L. on some pathogenic bacteria. Methods and Materials: In this descriptive-laboratory study, after collecting the plants and confirming its scientific name by botanists, methanolic extract of the plant was prepared by Soxhlet extractor method and different concentrations of extract were prepared. Then, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) extracts were determined on Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa using well-diffusion and dilution methods. Results: The results showed that MIC and MBC of methanol extract on Staphylococcus aureus, Bacillus cereus and Escherichia coli were 12.5, 25 and 50 mg / ml, respectively. Discussion and Conclusion: The results of this study show that Carum copticum L. plant can be considered in herbals groups with antibacterial properties. After evaluating their effects in vivo condition and identifying the active ingredients, it can be used as an alternative to synthetic drugs that commonly used to treat infections.